8ZXJ

Sweet protein MNEI-Mut 6-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.94138
Spacegroup name	P 21 21 21
Unit cell lengths	62.251, 49.737, 65.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.150 - 1.500
R-factor	0.1937
Rwork	0.192
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iv7
RMSD bond length	0.019
RMSD bond angle	1.590
Data reduction software	HKL-3000
Phasing software	PHENIX (1.19_4092)
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.150	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	33438	33311
<I/σ(I)>	13.2
Completeness [%]	99.5
Redundancy	4.2
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 6000