8ZXB
Structure of aminotransferase from Mycolicibacterium neoaurum in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.080, 79.080, 91.980 |
| Unit cell angles | 96.92, 108.47, 113.09 |
Refinement procedure
| Resolution | 28.400 - 1.750 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.224 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.740 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.056 | 0.192 |
| Rmeas | 0.067 | 0.244 |
| Rpim | 0.037 | 0.148 |
| Total number of observations | 487334 | 28522 |
| Number of reflections | 150805 | 10949 |
| <I/σ(I)> | 16.2 | 6.7 |
| Completeness [%] | 94.4 | |
| Redundancy | 3.2 | 2.6 |
| CC(1/2) | 0.995 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Sodium Cacodylate pH 6.5,0.2 M MgCl2,9 %PEG 3000 |
