8ZX7

Crystal structure of HCoV-OC43 fusion core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-06-11
Detector	BRUKER PHOTON 100
Wavelength(s)	0.9785
Spacegroup name	P 2 2 21
Unit cell lengths	39.031, 48.195, 103.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.190 - 3.340
R-factor	0.2769
Rwork	0.274
R-free	0.33490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	18.302
Data reduction software	HKL-3000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	3.360
High resolution limit [Å]	3.340	3.340
Number of reflections	2946	206
<I/σ(I)>	5.3
Completeness [%]	94.2
Redundancy	1
CC(1/2)	0.985	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	0.2 M ZnAc2, 0.1 M Sodium cacodylate pH 6.5, and 18% PEG 8K