8ZWY
Structure-Based Mechanism and Specificity of Human Galactosyltransferase B3GalT5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-05-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.645, 86.700, 86.996 |
| Unit cell angles | 90.00, 95.25, 90.00 |
Refinement procedure
| Resolution | 47.440 - 1.950 |
| R-factor | 0.2091 |
| Rwork | 0.208 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.532 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.086 | 0.362 |
| Number of reflections | 47592 | 3613 |
| <I/σ(I)> | 16.2 | 3.45 |
| Completeness [%] | 98.1 | 89 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M BIS-TRIS Propane pH8.5, 0.2M Potassium Sodium Tartrate, 20% w/v PEG 3350 |
