8ZWV

The Crystal Structure of carbonic anhydrase II from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	42.525, 72.204, 74.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.905 - 1.500
Rwork	0.152
R-free	0.18740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7onv
RMSD bond length	0.010
RMSD bond angle	1.879
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.530	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	37316	1847
<I/σ(I)>	15.2
Completeness [%]	100.0
Redundancy	8.4
CC(1/2)	0.999	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M CaCl2, 0.1M Hepes pH7.5, 30% PEG4,000