8ZWQ
pseudorabies virus dUTPase structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00830 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.249, 103.264, 98.733 |
| Unit cell angles | 90.00, 99.47, 90.00 |
Refinement procedure
| Resolution | 48.450 - 2.230 |
| R-factor | 0.2802 |
| Rwork | 0.277 |
| R-free | 0.33850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.519 | |
| Rmeas | 0.653 | |
| Rpim | 0.283 | |
| Number of reflections | 46889 | 4491 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.870 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.1 M Tris/HCl (pH 8.5) 2.0 M Ammonium hydrogenphosphate |
