8ZWN

Crystal structure of DNA-binding transcriptional activator EvgA from Escherichia coli str. K-12 substr. MG1655

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	164.378, 109.061, 106.666
Unit cell angles	90.00, 125.39, 90.00

Refinement procedure

Resolution	46.200 - 2.900
R-factor	0.2043
Rwork	0.199
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.555
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.200	3.026
High resolution limit [Å]	2.896	2.896
Rmerge	0.111	0.853
Rmeas	0.120	0.920
Rpim	0.046	0.344
Number of reflections	23189	1161
<I/σ(I)>	12.2	2.2
Completeness [%]	94.7	81.5
Redundancy	6.8	7.1
CC(1/2)	0.999	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	mixing 2 volumes of the protein (4 mg/ml in 20 mM Tris/HCl, pH 7.0, 200 mM NaCl, 1 mM TCEP) with 0.8 volume of the well solution (0.1 M Bis-Tris Propane, pH 8.5, 1.22 M Ammonium Tartrate) and 0.2 volume of 0.1 M Betaine.