8ZWD
Crystal structure of methanol dehydrogenase1 from Bacillus methanolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-13 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 256.022, 83.578, 220.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.130 - 3.000 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.676 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 167.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.200 | 0.386 |
| Rmeas | 0.242 | 0.449 |
| Rpim | 0.124 | 0.229 |
| Total number of observations | 17935 | |
| Number of reflections | 94482 | 4649 |
| <I/σ(I)> | 6.8 | 3.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.839 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium malonate, 0.1 M Bis Tris propane 7.2, 23 % w/v PEG 3350 |
