8ZVY
Alpha-Synuclein with H2a-H2b dimer complex structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.005, 68.240, 102.576 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.720 |
| R-factor | 0.1921 |
| Rwork | 0.191 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6w4l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.240 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 46279 | 2444 |
| <I/σ(I)> | 37.1 | 3.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.1 | |
| CC(1/2) | 1.000 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 100 mM Tris pH 8.0 and 10% PEG 8000. |
