8ZVU
Human citrate synthase intermediate 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.912, 59.689, 73.434 |
| Unit cell angles | 101.45, 98.91, 116.47 |
Refinement procedure
| Resolution | 50.980 - 1.780 |
| R-factor | 0.18573 |
| Rwork | 0.183 |
| R-free | 0.23311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.756 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.980 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.104 | 1.109 |
| Rmeas | 0.128 | 1.345 |
| Number of reflections | 71771 | 10449 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 90.3 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.968 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium acetate pH=7.1, 20% w/v PEG3350 |
