8ZVR
Human citrate synthase intermediate 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.924, 59.732, 73.280 |
| Unit cell angles | 101.58, 98.87, 116.40 |
Refinement procedure
| Resolution | 68.970 - 1.800 |
| R-factor | 0.22301 |
| Rwork | 0.220 |
| R-free | 0.27385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.727 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.970 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.140 | 1.163 |
| Rmeas | 0.180 | 1.508 |
| Number of reflections | 67914 | 9328 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 88.3 | |
| Redundancy | 5.3 | |
| CC(1/2) | 0.895 | 0.501 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium acetate pH=7.1, 20% w/v PEG3350 |
