Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.72 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.320, 55.970, 53.190 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 36.460 - 2.080 |
| R-factor | 0.1668 |
| Rwork | 0.160 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jym |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.101 | 0.488 |
| Number of reflections | 15371 | 2470 |
| <I/σ(I)> | 13.89 | 3.64 |
| Completeness [%] | 98.8 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.1 M Tris-HCl (pH 7.0 to 9.0) with 1.25 to 2.0 M ammonium sulfate and 12% (v/v) glycerol |
