8ZVJ

Crystal structure of VHL-EloB-EloC in complex with a fragment compound 7HC_1(DE22)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 41 2 2
Unit cell lengths	93.733, 93.733, 364.159
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.080 - 2.600
R-factor	0.2303
Rwork	0.228
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zrf
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	51235	2483
<I/σ(I)>	15.429
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)	1.000	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	12% PEG 8K, 0.1 M Na cacodylate-HCl pH 6.0, 0.2 M Mg acetate, 5 mM DTT