8ZV7

PARP15 catalytic domain in complex with RBN012759

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	49.753, 49.809, 157.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.880 - 1.900
R-factor	0.210581002302
Rwork	0.209
R-free	0.23946
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.063	0.449
Number of reflections	331136	31835
<I/σ(I)>	21.3
Completeness [%]	99.8
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	100mM HEPES buffer,pH6.5, 22%PEG 3350, 20% glycerol