8ZUS

Crystal structure of the F99S/M153T/V163A/T203V variant of GFP at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2024-02-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.70
Spacegroup name	P 21 21 21
Unit cell lengths	50.720, 62.236, 68.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.020 - 1.200
R-factor	0.1343
Rwork	0.133
R-free	0.16530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.984
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.020	1.270
High resolution limit [Å]	1.200	1.200
Rmerge	0.109	0.613
Rmeas	0.118	0.668
Number of reflections	68343	10858
<I/σ(I)>	10	2.12
Completeness [%]	99.9	99.6
Redundancy	6.73	6.34
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		308	PEG 4000, magnesium chloride, MES-NaOH buffer (pH 5.0)