8ZUR

Crystal structure of the F99S/M153T/V163A/T203V/E222Q variant of GFP at pH 5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2023-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.70
Spacegroup name	P 21 21 21
Unit cell lengths	51.500, 57.790, 68.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.200 - 1.200
R-factor	0.1305
Rwork	0.129
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.343
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.220	1.270
High resolution limit [Å]	1.200	1.200
Rmerge	0.087	0.798
Rmeas	0.094	0.869
Number of reflections	64730	9968
<I/σ(I)>	10.78	1.99
Completeness [%]	100.0	100
Redundancy	6.71	6.42
CC(1/2)	0.999	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		308	PEG 4000, magnesium chloride, MES-NaOH buffer (pH 5.0)