8ZUQ

Crystal structure of the F99S/M153T/V163A/T203I/E222Q variant of GFP at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	15
Detector technology	PIXEL
Collection date	2022-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.70
Spacegroup name	P 21 21 21
Unit cell lengths	50.498, 62.452, 67.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.930 - 1.480
R-factor	0.1578
Rwork	0.156
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.930	1.570
High resolution limit [Å]	1.480	1.480
Rmerge	0.160	0.784
Rmeas	0.174	0.847
Number of reflections	36411	5803
<I/σ(I)>	7.34	1.98
Completeness [%]	100.0	100
Redundancy	6.7	6.97
CC(1/2)	0.994	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		308	PEG 4000, magnesium chloride, MES-NaOH buffer (pH 5.0)