8ZUD

Crystal Structure of Human Myt1 Kinase domain Bounded with compound 8f

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-07
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	99.617, 55.863, 58.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.852 - 1.505
R-factor	0.204195659031
Rwork	0.203
R-free	0.23776
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1a
RMSD bond length	0.017
RMSD bond angle	1.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.853	1.559
High resolution limit [Å]	1.505	1.505
Number of reflections	52308	52308
<I/σ(I)>	0.86
Completeness [%]	100.0
Redundancy	8.7
CC(1/2)	0.910	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.8 M Ammonium sulfate,100 mM Sodium citrate,pH 6.5