8ZTQ

Crystal structure of Sufu from Mycoplasma Pneumonia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	149.080, 30.333, 87.209
Unit cell angles	90.00, 122.48, 90.00

Refinement procedure

Resolution	36.783 - 2.889
R-factor	0.2739
Rwork	0.271
R-free	0.29920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.889	7.860	2.900
Rmerge	0.198	0.099	0.780
Rmeas	0.217	0.110	0.875
Rpim	0.088	0.048	0.387
Number of reflections	7613	425	378
<I/σ(I)>	3.2
Completeness [%]	99.8	99.5	98.7
Redundancy	5.9	5.3	4.6
CC(1/2)	0.981	0.988	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	BIS-TRIS, PEG3350