8ZT4
Complex structure of N-acetyltransferase SbzI and carrier protein SbzG in the biosynthesis of altemicidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.150, 95.680, 130.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.600 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 1.168 |
| Number of reflections | 30470 | 3652 |
| <I/σ(I)> | 23.3 | 4.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18 | 18.9 |
| CC(1/2) | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 6.5, 0.2 M KSCN, 15% PEG 8000, 3% ethylene glycol |
