8ZT4

Complex structure of N-acetyltransferase SbzI and carrier protein SbzG in the biosynthesis of altemicidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	77.150, 95.680, 130.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.840 - 2.600
R-factor	0.1984
Rwork	0.196
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.005
RMSD bond angle	0.707
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.120	1.168
Number of reflections	30470	3652
<I/σ(I)>	23.3	4.5
Completeness [%]	99.9	99.9
Redundancy	18	18.9
CC(1/2)		0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES, pH 6.5, 0.2 M KSCN, 15% PEG 8000, 3% ethylene glycol