8ZS0

Plasmodium falciparum Nucleoplasmin (PfNPM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 31 2 1
Unit cell lengths	89.349, 89.349, 113.282
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.700 - 3.250
R-factor	0.226
Rwork	0.224
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.896
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.280	3.510
High resolution limit [Å]	3.250	3.250
Number of reflections	15903	1726
<I/σ(I)>	10.6	2.2
Completeness [%]	99.9
Redundancy	24.8
CC(1/2)	0.991	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	0.2 M sodium malonate. 20% PEG3350