8ZR7

Crystal structure of Skd3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 65
Unit cell lengths	119.230, 119.230, 90.589
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.855 - 3.000
R-factor	0.2122
Rwork	0.210
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.349
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.049	0.033	0.359
Rmeas	0.052	0.035	0.394
Rpim	0.014	0.009	0.155
Total number of observations	158169
Number of reflections	16188	850	765
<I/σ(I)>	11.9
Completeness [%]	98.2	98.4	95.6
Redundancy	9.8	14.4	5.4
CC(1/2)	1.000	1.000	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% SOKALAN cp5, 100 mM Bis-Tris pH 6.0, 100 mM ammonium sulfate