8ZR0

Crystal structure of Skd3 in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 65
Unit cell lengths	119.699, 119.699, 89.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.833 - 2.900
R-factor	0.2074
Rwork	0.205
R-free	0.25470
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.329
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.053	0.034	0.298
Rmeas	0.056	0.035	0.330
Rpim	0.016	0.009	0.138
Number of reflections	16197	850	751
<I/σ(I)>	12.7
Completeness [%]	98.8	99.2	94.5
Redundancy	9.7	14.4	4.8
CC(1/2)	1.000	0.999	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% SOKALAN cp5, 100 mM Bis-Tris pH 6.0, 100 mM ammonium sulfate