8ZQI

Glucose bound Toxascaris leonina galectin (Tl-gal) W77F/W212F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-11-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	78.478, 81.378, 112.324
Unit cell angles	90.32, 89.91, 90.37

Refinement procedure

Resolution	34.350 - 2.980
R-factor	0.1973
Rwork	0.193
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.272
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.900	2.970
Rmerge	0.088	0.088
Number of reflections	55160	55160
<I/σ(I)>	16.44	16.44
Completeness [%]	98.2
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% polyethylene glycol 8000, 100mm sodium/potassium phosphate (pH 6.2), 200mm NaCl