8ZQG

Crystal structure of WIPI3 in complex with ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 63
Unit cell lengths	182.663, 182.663, 54.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.870 - 2.770
R-factor	0.192
Rwork	0.190
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyy
RMSD bond length	0.008
RMSD bond angle	1.216
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.330	2.820
High resolution limit [Å]	2.770	2.770
Rpim	0.036	0.435
Number of reflections	26763	1330
<I/σ(I)>	16.1
Completeness [%]	100.0
Redundancy	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	289	0.02 M Citric acid, 0.08 M BIS-TRIS propane (pH 8.8); 16% w/v Polyethylene glycol 3350