8ZQ7

Crystal structure of prefusion RSV F protein

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	158.423, 168.696, 190.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	126.350 - 2.770
R-factor	0.2076
Rwork	0.207
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.350	2.870
High resolution limit [Å]	2.770	2.770
Rmerge	0.085	0.681
Number of reflections	99576	899
<I/σ(I)>	7.94
Completeness [%]	76.7
Redundancy	2
CC(1/2)	0.989	0.291

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8% Tacsimate pH6.0+20% PEG 3350