8ZPU

Crystal structure of the anti-phosphorylated peptide C7 scFv antibody with peptide bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	126.610, 126.610, 82.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.100 - 2.800
Rwork	0.304
R-free	0.34520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.371
Data reduction software	MOSFLM (7.4.0)
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.100	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.176	1.030
Rpim	0.055	0.321
Number of reflections	19037	2756
<I/σ(I)>	12.8	2.6
Completeness [%]	99.8
Redundancy	11.1
CC(1/2)	0.996	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Ammonium sulfate, 30% Polyethylene glycol 4000, 20 mM Tris HCl, 20 mM NaCl, pH 8.0