8ZPQ

Crystal structure of SARS-Cov-2-BQ1.1-RBD and 70fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-01-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.978510
Spacegroup name	P 21 21 21
Unit cell lengths	69.886, 93.251, 257.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.350 - 2.750
R-factor	0.2362
Rwork	0.234
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.920
High resolution limit [Å]	2.750	2.750
Number of reflections	43657	7119
<I/σ(I)>	15.47	2.01
Completeness [%]	97.6	99.9
Redundancy	13.05
CC(1/2)	0.998	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Na HEPES, pH7.0, 10% w/v PEG6000