8ZPA

Crystal structure of a HSA/probe complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	185.705, 38.441, 93.290
Unit cell angles	90.00, 103.92, 90.00

Refinement procedure

Resolution	37.040 - 2.590
R-factor	0.2401
Rwork	0.237
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.501
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.491	2.550
Number of reflections	21415	2078
<I/σ(I)>	10.6
Completeness [%]	99.5
Redundancy	6.78
CC(1/2)	0.996	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	315	30% polyethylene glycol monomethyl ether 550, 0.1 M HEPES pH 7.5, 50 mM MgCl2