8ZOZ

Crystal structure of the complex of glyceraldehyde-3-phosphate dehydrogenase of type B from Acinetobacter baumannii with Adenosine monophosphate at 3.20 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	298
Detector technology	PIXEL
Collection date	2023-07-28
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87313
Spacegroup name	C 2 2 21
Unit cell lengths	147.509, 167.732, 150.726
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.007 - 3.200
Rwork	0.201
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.260	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.230	2.390
Rmeas	0.240	2.510
Rpim	0.069	0.757
Number of reflections	30330	4487
<I/σ(I)>	7.9	1.1
Completeness [%]	97.2	99.7
Redundancy	12.8	10.9
CC(1/2)	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	298	0.2M MgSo4. 6H2O, 0.1M HEPES sodium (pH 7.5)