8ZOJ

The crystal structure of YegTK267A from the Nucleoside: H+ Symporter Family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	42.769, 58.212, 105.076
Unit cell angles	77.85, 81.30, 68.46

Refinement procedure

Resolution	39.139 - 2.913
R-factor	0.2434
Rwork	0.241
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.378
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	30.000	3.090
High resolution limit [Å]	2.910	8.590	2.910
Rmerge	0.078	0.029	1.594
Rmeas	0.111	0.040	0.929
Number of reflections	20090	182	1006
<I/σ(I)>	4.03
Completeness [%]	98.1
Redundancy	3.43
CC(1/2)	0.996	0.996	0.127

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.1 M MES (pH=5.8), 0.1 M Li2SO4, 0.1 M glycine, and 26% PEG550MME