8ZO4

Crystal structure of Tfh TCR ectodomain thermostable mutant

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-05-15
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0520
Spacegroup name	P 32 2 1
Unit cell lengths	89.156, 89.156, 208.683
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.580 - 2.600
R-factor	0.2285
Rwork	0.226
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.580	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.108	1.054
Rpim	0.035	0.349
Number of reflections	303548	30201
<I/σ(I)>	17.95
Completeness [%]	99.9	99.97
Redundancy	10	10
CC(1/2)	0.998	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Bis-tris (pH 5.5), 0.2M Potassium sulfate, 25% PEG 3350