8ZNS

Type I-C CRISPR-associated protein, Cas3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2023-06-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	124.970, 73.330, 104.420
Unit cell angles	90.00, 99.75, 90.00

Refinement procedure

Resolution	29.090 - 2.170
R-factor	0.1972
Rwork	0.195
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.090	2.248
High resolution limit [Å]	2.170	2.170
Number of reflections	49372	4905
<I/σ(I)>	12.84
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.999	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% (w/v) PEG 8000, 0.1M Na/K phosphate pH 6.2, 0.2M NaCl