8ZNM
CRYSTAL STRUCTURE OF DI-C-GLYCOSYLTRANSFERASE GGCGT MUTANT IN COMPLEX WITH UDP-GLC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-05 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.618, 81.849, 94.141 |
| Unit cell angles | 64.76, 88.28, 88.24 |
Refinement procedure
| Resolution | 29.640 - 2.210 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.571 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 2.289 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.059 | 0.496 |
| Rmeas | 0.070 | 0.593 |
| Rpim | 0.037 | 0.320 |
| Number of reflections | 98869 | 9933 |
| <I/σ(I)> | 7.54 | |
| Completeness [%] | 96.8 | 97.15 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.997 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M Sodium chloride, 0.1 M Tris pH7.5, 22.5% w/v Polyethylene glycol 3,350 |
