8ZNA
Myxococcus xanthus RomX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.295, 56.076, 68.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.955 - 1.888 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.23080 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.044 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.955 | 9.060 |
| High resolution limit [Å] | 1.800 | 1.890 |
| Rmerge | 0.115 | 0.055 |
| Rmeas | 0.120 | 0.057 |
| Rpim | 0.032 | 0.015 |
| Number of reflections | 19678 | 128 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.1 | 98.9 |
| Redundancy | 19.8 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 25% PEG MME 550, 15 mM MgCl2, 40 mM HEPES Na pH=7 |
