8ZN0
The Crystal Structure of an Atypical N-methyltransferasea PaOMT9 in P. amurense
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-04 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 43 |
| Unit cell lengths | 66.346, 66.346, 167.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.910 - 1.800 |
| R-factor | 0.2167 |
| Rwork | 0.161 |
| R-free | 0.20440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.901 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.21.1_5286) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.910 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.128 | 1.327 |
| Rmeas | 0.139 | 1.429 |
| Rpim | 0.053 | 0.573 |
| Number of reflections | 66419 | 3846 |
| <I/σ(I)> | 14.4 | 2.2 |
| Completeness [%] | 99.9 | 98.1 |
| Redundancy | 13.5 | 12.7 |
| CC(1/2) | 0.998 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 20% w/v PEG1000, 100 mM lithium sulfate monohydrate and 100 mM sodium citrate tribasic dihydrate pH 5.5 |
