8ZMW

Structure of a triple-helix region of human Collagen type VII from Trautec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	20.927, 30.425, 53.078
Unit cell angles	78.40, 81.64, 72.83

Refinement procedure

Resolution	28.659 - 1.750
Rwork	0.133
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.798
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.660	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	11871	607
<I/σ(I)>	15.3
Completeness [%]	96.6
Redundancy	3.4
CC(1/2)	0.999	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Crystal-B6 (0.2M MgAc2, 0.1M Sodium cacodylate pH6.5, 20% PEG 8000)