8ZMV

Structure of a triple-helix region of human Collagen type XVII from Trautec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95300
Spacegroup name	P 1 21 1
Unit cell lengths	33.960, 13.757, 66.084
Unit cell angles	90.00, 100.12, 90.00

Refinement procedure

Resolution	32.530 - 1.450
Rwork	0.230
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.530	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	11135	546
<I/σ(I)>	15.2
Completeness [%]	98.7
Redundancy	6.5
CC(1/2)	0.998	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Index-H6 (0.2M Sodium formate, 20% PEG 3350)