8ZMV
Structure of a triple-helix region of human Collagen type XVII from Trautec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-03-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.960, 13.757, 66.084 |
Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
Resolution | 32.530 - 1.450 |
Rwork | 0.230 |
R-free | 0.29840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.573 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.530 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 11135 | 546 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 98.7 | |
Redundancy | 6.5 | |
CC(1/2) | 0.998 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Index-H6 (0.2M Sodium formate, 20% PEG 3350) |