8ZMU

GLUTAMATE DEHYDROGENASE (W89F-MUTANT) FROM THERMOCOCCUS PROFUNDUS IN THE UNLIGANDED STATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-09
Detector	RAYONIX SX-165mm
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	113.355, 163.248, 132.605
Unit cell angles	90.00, 113.91, 90.00

Refinement procedure

Resolution	21.800 - 2.028
R-factor	0.17357
Rwork	0.172
R-free	0.19944
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.766
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.028	2.028
Rmerge	0.063	0.293
Number of reflections	283125	281936
<I/σ(I)>	18.3
Completeness [%]	99.8
Redundancy	3.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	293	1.6 M lithium sulfate (Wako, Osaka, Japan), 1.7%(w/v) poly-ethylene glycol 8000 (PEG800) (Sigma-Aldrich, St. Louis, USA) and 0.1 M sodium acetate (Wako)