8ZMM

Structure of a triple-helix region of human Collagen type IV from Trautec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95300
Spacegroup name	P 1 21 1
Unit cell lengths	30.479, 26.425, 67.617
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	33.380 - 1.300
Rwork	0.144
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.690
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.380	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	26186	1184
<I/σ(I)>	9.7
Completeness [%]	98.8
Redundancy	6.4
CC(1/2)	0.993	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Index-E7 (0.05M MgCl2, 0.1M HEPES pH7.5, 30% PEG 550 MME)