8ZLZ

The complex crystal structure of CcmS and C-terminus of CcmK1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	67.493, 67.493, 118.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.010 - 1.670
R-factor	0.1845
Rwork	0.182
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.145
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.600	1.760
High resolution limit [Å]	1.670	1.670
Number of reflections	32268	4199
<I/σ(I)>	27.8
Completeness [%]	98.5
Redundancy	22
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Lithium sulfate, 0.1 M TRIS pH8.5, 1.26 M Ammonium sulfate