8ZLX
Crystal Structure of mPPEF2 IQ motif/apo-CaM Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 43 |
| Unit cell lengths | 134.451, 134.451, 35.944 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.500 |
| R-factor | 0.2124 |
| Rwork | 0.208 |
| R-free | 0.29083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.830 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.151 | 0.959 |
| Number of reflections | 22846 | 22846 |
| <I/σ(I)> | 17.82 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 289 | 1.4 M Sodium citrate tribasic dihydrate pH 6.1 |
