8ZLV
Asymmetric Dimeric Structure of the Catalytic Domain in Truncated Threonine Deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.159, 102.159, 116.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.690 - 2.900 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tdj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.226 | 1.744 |
| Rmeas | 0.232 | 1.787 |
| Rpim | 0.051 | 0.386 |
| Number of reflections | 14200 | 1409 |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20.3 | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M CaCl2H4O2, 0.1 M HEPES sodium pH 7.5 and 28% v/v PEG 400 |
