8ZLK

PWWP domain from human DNMT3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2024-03-22
Detector	RAYONIX MX300HE
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	73.940, 73.940, 156.973
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.190 - 2.740
R-factor	0.2256
Rwork	0.221
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.720
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.840
High resolution limit [Å]	2.740	5.890	2.740
Rmerge	0.060	0.030	0.496
Rmeas		0.031	0.523
Rpim		0.009	0.162
Number of reflections	13596	1484	1346
<I/σ(I)>	20.2
Completeness [%]	100.0	99.7	100
Redundancy	10	10.6	10.1
CC(1/2)	0.994	1.000	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	Lithium sulfate monohydrate, CAPS, Ammonium sulfate