8ZLF

Crystal structure of DH domain of FYVE Domain containing protein(FP10) from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	56.590, 69.337, 57.615
Unit cell angles	90.00, 118.28, 90.00

Refinement procedure

Resolution	50.741 - 2.480
Rwork	0.234
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.423
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	BALBES
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.741	5.544
High resolution limit [Å]	2.480	2.480
Number of reflections	16337	1780
<I/σ(I)>	2.6
Completeness [%]	90.4
Redundancy	7.1
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	1.5M Sodium Citrate tribasic dihydrate, 0.1M Sodium Citrate pH 6.5