8ZLD
Crystal structure of PfHO from Plasmodium falciparum at 2.78 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-21 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.910, 55.370, 162.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.790 - 2.780 |
R-factor | 0.20539 |
Rwork | 0.203 |
R-free | 0.26366 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.559 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.400 | 2.930 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.107 | 0.715 |
Rpim | 0.045 | |
Number of reflections | 13064 | 1843 |
<I/σ(I)> | 11.2 | 2.3 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.6 | 6.4 |
CC(1/2) | 0.998 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.4M (NH4)2SO4, 0.65M Li2SO4, 0.1M sodium citrate tribasic dihydrate, pH 5.6 |