8ZLC

Crystal Structure of Ankyrin Repeat Protein from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	42.377, 62.742, 91.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.797 - 2.130
Rwork	0.185
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.539
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.800	2.185
High resolution limit [Å]	2.130	2.130
Number of reflections	18741	910
<I/σ(I)>	5.35
Completeness [%]	99.9
Redundancy	4.82
CC(1/2)	0.994	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2M Ammonium Acetate, 25% PEG 3350, 0.1M bis-tris pH 5.5