8ZLA

Crystal Structure of monomeric Rag-like small GTPase from Asgard Lokiarchaeota (LokiRagM) in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-23
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000032
Spacegroup name	P 41 2 2
Unit cell lengths	85.040, 85.040, 153.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 1.750
R-factor	0.1752
Rwork	0.173
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.382
Data reduction software	DIALS (3.18.0)
Data scaling software	DIALS (3.18.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.040	1.770
High resolution limit [Å]	1.730	1.730
Rmerge	0.075	1.266
Rpim	0.021	0.405
Number of reflections	57509	49830
<I/σ(I)>	25	2.5
Completeness [%]	97.6	81.4
Redundancy	26.1	19.4
CC(1/2)	0.999	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	PEG 8000, ethylene glycol, magnesium chloride, calcium chloride