8ZKJ
The crystal structure of apo-LDHC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 86.000, 86.000, 194.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.050 - 2.380 |
| R-factor | 0.2117 |
| Rwork | 0.208 |
| R-free | 0.26130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7epm |
| RMSD bond angle | 14.644 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.050 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 770756 | 2181 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.5 | |
| CC(1/2) | 0.997 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M ammonium sulfate |
