8ZJB

Oomycete Nudix effectors display WY-Nudix conformations with mRNA decapping activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9864
Spacegroup name	P 21 21 21
Unit cell lengths	73.364, 164.496, 169.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.350 - 3.190
R-factor	0.2587
Rwork	0.256
R-free	0.31080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.363
Data reduction software	DIALS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.190	3.190
Rmerge	0.139	1.240
Rmeas	0.154
Rpim	0.065
Number of reflections	34822	1668
<I/σ(I)>	21.8
Completeness [%]	99.8	100
Redundancy	6	6.5
CC(1/2)	0.978	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	277	0.2 M sodium citrate trisbase dihydrate (pH 8.3), 20% (w/v) PEG 3350